Screening Some Medicinal Plants Against Moroccan Watermelon Mosaic Virus (MWMV)

Project and Seminar Material for Biochemistry

Screening Some Medicinal Plants Against Moroccan Watermelon Mosaic Virus (MWMV)


Abstract


The antiviral activities of plant leaves extract of four species, were investigated. The extract were tested for antiviral activities on the host plant (Cocumba) against Moroccan watermelon mosaic virus. Results showed that two plants (Phyllanthus amerus and Mirabiles jalapa) presented inhibitory activities against the virus. While Ficus exaspirata and Citrus spp were unable to inhibit the virus.

Moroccan watermelon mosaic virus is still a major problem on the cucubite cultivation in Africa.


Chapter One


Introduction

1.1 Background of Study

Plant viruses are responsible for huge economic losses in many countries around the world.

A virus is an infection agent that typically consists of nuclei and molecule in a protein coat, it is too small to be seen by light microscopy, and is able to multiply only within the living cells of a host. (Holmes, 1939). Viruses can be spread by direct transfer of sap by contact of a wounded plant with a healthy one, such contact may occur during agricultural practices, as by damage caused by tools or hands, or naturally as by animal feeding on the plant. Most of the viruses infecting plants rely on insects to move from one host to another, some remain associated with the mouth parts and can be inoculated within seconds or minutes. (Martinus, 1898). This work those not cover insect transmission but basically mechanical inoculation.


1.2 Methods Employed to Control Plant Viruses

Plant viruses and virus diseases have been studied for more than 100 years and much attention has been given to their control. However, this has been difficult to achieve because of the lack of any effective means of curing virus-infected plants. Chemotherapy, thermotherapy and Meristem-tip culture can be successful but they cannot be used on a large scale. (Brook, 1964). The main approach has been to prevent or delay virus infection or to minimize its effect. Various means have been used to achieve these objectives, including phyto-sanitation. (Involving quarantine measures, crop hygiene use of virus-free plant materials and eradication) changes in crop practices, use of pesticide for control of vectors, mild strain protection and the employment of resistant or tolerant varieties. (Sarkar, 1995). Some viruses can be eliminated from infected plant by heat or meristem-tip therapy or by the use of chemicals (Faccioli and Marami, 1998) these methods are used widely to develop virus-free plants of vegetatively-propagated crops for further propagation. It prevents plants from becoming infected, delay infection to such a life stage of crop impaired and decrease the effects of infection. (Mink et al, 1998).

1.2.1 Ricinus Sp (Castor Oil Plant)

Ricinus Sp (Castor oil plant) has being classified as a member of the sponge family, Euphobiaceae. The seed from Ricinus sp plant contain in excess of 45% oil. The said oil is used widely for various purposes. It is used as a lubricant, in high speed engine and aeroplanes, in the manufacture of soap, transparent paper, printing ink, varnishings, linolilium and plasticizer. It is also used for medical and lighting purposes. It has antimicrobial activities against gram positive bacteria (Nuttall & Labuda, 2008).

1.2.2 Mirabilis Jalapa. (The Four 0’clock Plant)

Mirabilis Jalapa. (The four 0’clock plant) has being classified as a member of the Nyctaginaleae family the species mirabilis Jalapa is a commonly grown ornamental plant and is available in a range of colours. The flower of Mirabilis Jalapa is used for food colouring, and the leaves may be eaten cooked as food. It serves as emergency food. It is used for dye production for cakes and jellies. It is also used for cosmetics production. It has antiviral protein (MAP) which was demonstrated to possess abortificiant actively in pregnant mice, inhibitory effect on call-free protein synthesis and antiproliferative effect on tumor cells. (Wong et al, 2014).

1.2.3 Phyllanthus Amerus

Phyllanthus amerus is a member of the Euphorbiaceae family, it is commonly called the stone breaker, the plant extract from Phyllanthus according to (Nicole, 1998) has being used for killing bacteria, expels stones, support kidneys and treat malaria.

1.2.4 Adansonia Digitata

Adansonia Digitata belongs to the family Malvaceae. This species is found in hot, dry savannahs’ of Sub-Saharan Africa. Common names, Baobab, monkey bread tree, dead-rat tree and cream of taster tree etc. The leaves are used either fresh as a cooked vegetable or dried and powered as an ingredient of soups and sauces. The shoots and roots of seedlings are eaten as well. The roots are boiled and eaten in West Africa in times of famine. It is used as functional food for the well being of the rural communities, food for livestock, shelter for the living and the dead, the bark of a digitata has been imported in the past into Europe by the packing and paper industry and for medicinal use. Under the name cortex Cael Cedra. It was used as a substitute for quinine to reduce fever (Jardin, 1968).

1.2.5 Acalypha Indica

Acalypha Indica belong to the family Euphorbialeae. It is a common herb growing up to 75cm tall with ovate leaves. The leaves are cooked and eaten as vegetable. This plant is held to high esteem in traditional medicine, as it is believed to rejuvenate the body. It is useful bronchitis pneumonia, ashma and pulmonary tuberculosis. It also has significant antibacterial and antifungal activities, both against human and plant pathogen. (Burkill, 1974).


1.3 Plant as Reserviour of Antiviral substance

Plants from Northern Nigeria with a history of use in both human and veterinary traditional medicine have been investigated for their antiviral activity and their hypotoxility determined. Most of the extracts have activity against more than one virus of a dose rate of between 100 and 400 microg/100 microl. (Mehesh, 2000).

Lawsonia Inermis: Popularly known as Hemma or mehindi in the oriental world, is an evergreen medium sized shrub belonging to the family hythraccere. This plant harbors a well documented folklore history for treating convulsion, jaundice and malignant ulcers. Phytochemical studies in henna plant have indicated the presence of several bioactive molecular like isophumpagin, hipeol, 30-norlupan -3-01-20-one betuhennan, betuhennamic acid and nstigmasterol in leaves and roots. Plant extract have been known to be depressing antimicrobial, antioxidant, wound healing, anti-inflammatory antipyretic, analgesis actions, (Keyvan, 2008).


1.4 Morocan Water Melon Mosaic Virus

The moroceam water melon mosaic virus (MWMV), has been determined to be a distinct members of the polyvirus group.

(Cockerhem, 1970) the relationship of the morocean water melon mosaic virus and other polyviruses and the W strain of papaya rignspoot virus, was examined by comparing tryptic peptide profiles using high performance liquid chromatography. The profolus indicated that the low protein sequence of MWMV differed substantially from those of the other poly-viruses. (McKern, 1993).


Chapter Five


Discussion and Conclusion

5.1 Discussion

The leaves of the cucumber plant that were inoculated with Phyllanthus amerus and Mirabilis jalapa did not show symptoms of infections from the Moroccan watermelon mosaic virus ,which were found to be similar to those obtained by other workers ( Essam et al,2014 , Owolabi et al 2012 ). Based on this result we may postulate that extract from P. amerus and M. jalapa can be used as antiviral agents against (MWMV).

The extract from F . exasperate and Citrus spp were unable to inhibite the virus. All plants that were inoculated with the virus and F. exasperata and Citrus spp as antiviral agents show symptoms of infection.

The effect of P. amerus and M. jalapa on the virus may be due to the to its high content of tannins. It was mentioned that it contained 50 – 70 % of tannin ( Al- shamma, 1989).

Similar results were obtained by several workers in using different plant extracts on many viruses ( Al – Ani and Hassan, 2002, Al-Azawi et al, 2008). The percentage of inhibition comparing to other workers may be due to the method used.

The extracts from F. exasperate and Citrus spp on the infectivity of Moroccan watermelon mosaic virus was abortive because the extracts from these plants could not inhibite the viral infection on the cucumber plant.


5.2 Conclusion

In conclusion Moroccan watermelon mosaic virus is still a major problem on the cucubite cultivation in Africa . the strategy is to restrict the use of pesticides in the management program of the disease, because the misuse of some insecticides had caused serious problem to farmers. This research was directed to find plant extract of several plants which can inhibite the Moroccan watermelon mosaic virus and so far it has proved that P. amerus and M. jalapa has proved effectivity against the virus . therefore, P. amerus and M. jalapa extracts can be used for the virus prevention, but more work is needed.


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