The Role Of N-Butanol Leaf Fraction Of Telfairia Occidentalis On Some Haematological Parameters In Phenyl-Hydrazine Induced Anemia In Wistar Rats

Project and Seminar Material for Physiology

Project and Seminar Material for Physiology


Abstract


Anemia is a serious health problem especially in developing countries. In the tropics, rural dwellers had resort to herbal treatment in some cases of anemia. The main aim of this work was to ascertain the role of n-butanol leaf fraction of Telfairia Occidentalis on some haematological parameters in phenyl hydrazine induced anemia in wistar rats. Thirty (30) adult Wistar rats were used for this study. All animals were assayed for haematological parameters 1day before the onset of the experiment. The animals were grouped into five groups of five rats each (n =5). Anemia was induced in the Wistar rats by intraperitoneal injection of Phenyl hydrazine Hydrochloride 50mg/kg w/w in DSMO once daily for 3 days.

Group i. serves as the negative control group, received 50mg/kg of Phenylhydrazine Hydrochloride and 1ml/kg normal saline (untreated) group ii. serve as Positive control group received Vitamin B12 0.4ml/kg (standard haematinic) intramuscularly,while group iii, iv. and v. received 100mg/kg, 200mg/kg and 300mg/kg body weight of n-butanol leaf fraction of Telfairia occidentalis for 2 weeks respectively. Asessment of RBC and WBC was carried out using the newly improved Neubauer counting chamber, PCV using the microhaematocrit reader, Hb using the AO-Hb meter and Bilirubin concentration using colorimetric estimation for the serum bilirubin.

There was a significant (P6/ʯL as compared to 4.78±0.17×106/ʯL), PCV (50.60±0.51% as compared to 37.08±0.37%), HB (16.84±0.18 g/dl as compared to 12.56±0.13 g/dl), WBC (6.10±0.15 x109/L as compared to 6.17±0.88×109/L) and Bilirubin (18.16±0.08a ʯmol/L as compared to 15.54±0.21 ʯmol/L). However, the significant increase obtained from the results of RBC ,PCV and Bilurubin was not dose dependent while that of HB and WBC was dose dependent.

In conclusion, Intraperitoneal administration of 50mg/kg Phenylhydrazine hydrochloride for 3days decreases the blood parameters below the pre-anemic level (1day before induction) due to the production of reactive oxygen species. Phytochemical screening of this leaf fraction indicates the presence of flavonoids, saponin secoiridoid glycosides and alkaloids, these natural antioxidants could be responsible for reversing the damaging effect of PHZ and thus playing a modulatory role and also maintaining the integrity of the RBC.


Chapter One


1.0 Introduction

Anemia is a global public health problem affecting both developing and developed countries with major consequences for human health as well as social and economic development (WHO, 2005).

Anemia is a reduction from the normal quantity of circulating hemoglobin in the blood less than 13 g/dl for male and less than 12 g/dl for female adults (Okochi et al., 2003). It occurs at all stages of the life cycle, but is more prevalent in pregnant women and young children (Adam et al., 2005).

Hemolytic anemia is a form of anaemia due to hemolysis, the abnormal breakdown of Red Blood Cells (RBCs), either in the blood vessels (intravascular hemolysis) or elsewhere in the human body (extravascular). It has numerous possible causes, ranging from relatively harmless to life-threatening (Telford et al., 2003). The general classification of hemolytic anemia is either inherited or acquired. Treatment depends on the cause and nature of the breakdown (Yamoto et al., 1998).

Anemia is the result of a wide variety of causes that can be isolated, but more often coexist. Globally, the most significant contributor to the onset of anemia is iron deficiency so that IDA and anemia are often used synonymously, and the prevalence of anemia has often been used as a proxy for IDA (De Maeyer, 1989).

It is generally assumed that 50% of the cases of anemia are due to iron deficiency, but the proportion may vary among population groups and in different areas according to the local conditions (De Maeyer, 1989)..


The Role Of N-Butanol Leaf Fraction Of Telfairia Occidentalis On Some Haematological Parameters In Phenyl-Hydrazine Induced Anemia In Wistar Rats


Project Material Download

3,000 Naira


The complete material will be sent to you in just 2 steps.

Quick & Simple…


Step One Purchase

Make payment of ₦3,000: through USSD Transfer, Bank Mobile App, ATM Transfer, or POS Transfer to:

Access Bank PlcAccount No.: 0811003731
Name: Samphina Academy
Account Type: Current

Or Click Here to pay with Debit Card

FOR CLIENTS OUTSIDE NIGERIA:
Click Here to pay with Debit Card ($15)
GHANA – Make Payment of 60 GHS to MTN MoMo, 0553978005, Douglas Osabutey 

  PAY WITH CRYPTOCURRENCY


Step Two Purchase

Send the following details through Text Message or WhatsApp Messenger | +234-8143831497

  • Payment Details 
  • Email Address 
  • The Role Of N-Butanol Leaf Fraction Of Telfairia Occidentalis On Some Haematological Parameters In Phenyl-Hydrazine Induced Anemia In Wistar Rats

The complete material will be sent to your email address after receiving your payment information | T & C Apply


  Contact Our Help Desk


You may also like:

⚠️ Need a different topic? Perform a quick search



Get A Complete Business Plan For Any Business In Nigeria

Business Plan for Businesses in Nigeria

  Business Plans in Nigeria


The Role Of N-Butanol Leaf Fraction Of Telfairia Occidentalis On Some Haematological Parameters In Phenyl-Hydrazine Induced Anemia In Wistar Rats


Disclaimer

This research material “The Role Of N-Butanol Leaf Fraction Of Telfairia Occidentalis On Some Haematological Parameters In Phenyl-Hydrazine Induced Anemia In Wistar Rats” is for research purposes and should be used as a guide in developing your research project / seminar work. For no reason should you copy word for word (verbatim) as samphina.com.ng will not be liable for any who copied the material.

The aim of providing this material is to reduce the stress of moving from one school library to another all in the name of searching for research materials. This service is legal because, all institutions permit their students to read previous projects, books, articles or papers while developing their own works. According to Austin Kleon “All creative work builds on what came before”.

samphina.com.ng is only providing this material “The Role Of N-Butanol Leaf Fraction Of Telfairia Occidentalis On Some Haematological Parameters In Phenyl-Hydrazine Induced Anemia In Wistar Rats” as a reference for your research. The paper should be used as a guide or framework for your own paper. The contents of this paper should be able to help you in generating new ideas and thoughts for your own research. Use it as a guidance purpose only.


How to defend your research work


This is a general guide on how to defend your research work:

1. Prepare For Questions:

If you are preparing for questions that may be asked during your defense, then your answers will flow smoothly and effectively. This will prove your knowledge on the subject e.g “The Role Of N-Butanol Leaf Fraction Of Telfairia Occidentalis On Some Haematological Parameters In Phenyl-Hydrazine Induced Anemia In Wistar Rats“, and strengthening your argument. Ask friends and family, read your work for them to listen to your presentation, and write down questions. You may be lucky the panel will ask you those you have already prepared on.

2. Strong Summary:

Summarizing your chapters will help keep your audience focused because it is easy for a mind to drift, so providing summaries will ensure your panel will follow along, even if they lose focus for a brief moment. Visual aides, such as graphs and power-point presentations can be very helpful. If you are going to use these, make sure you will practice your presentation with them.

3. Be Confident in Your Research Work:

Not knowing your topic “The Role Of N-Butanol Leaf Fraction Of Telfairia Occidentalis On Some Haematological Parameters In Phenyl-Hydrazine Induced Anemia In Wistar Rats” inside out will cause you to struggle and ultimately fail with your defense. You need to know the subject from every angle to ensure you are fully prepared for any question that may come your way.

4. Conclusion:

Reinforce your findings to conclude your defense. The finale of your presentation should focus on proving the work that has been done. You may need to recap on what has changed and remained unchanged, if is necessary.

5 . Listen:

Before you get defensive or recite a particular answer, make sure you truly understand the question being asked. Being a good listener is an important quality, because providing an inaccurate or off-topic answer will also weaken the validity of your paper.

Samphina Academy

Samphina Academy is an Online Educational Resource Center that is aimed at providing students with quality information and materials to aid them in succeeding in their academic pursuit.