The Phytochemical Constituents Of Hexanolic Extract Of Ocimum Gratissimum (Scent Leaves)

Project and Seminar Material for Biochemistry

The Phytochemical Constituents Of Hexanolic Extract Of Ocimum Gratissimum (Scent Leaves)


Table of Contents


  • Title page i
  • Certification page ii
  • Dedication iii
  • Acknowledgement iv
  • Abstract v
  • Table of content

Chapter One

  • 1.0 Introduction
  • 1.1 Aim of Study
  • 1.2 Literature Review
  • 1.2.1 Phytochemical Screening
  • 1.2.1.1 Flavonoid
  • 1.2.1.2 Saponin
  • 1.2.1.3 Tannin
  • 1.2.1.4 Alkaloids

Chapter Two

  • 2.0 Materials and Methods
  • 2.1 Materials Used
  • 2.2 Reagents
  • 2.3 Identification and Preparation of plant materials
  • 2.4 Phytochemical analysis of hexanolic extract of O. gratissimum
  • 2.4.1 Qualitative determination
  • 2.4.1.1 Test for Alkaloids
  • 2.4.1.2 Test for Flavonoids
  • 2.4.1.3 Test for Saponin
  • 2.4.1.4 Test for Phytosterol
  • 2.4.1.5 Test for Phenol
  • 2.4.1.6 Test for Steroids
  • 2.4.1.1 Test for Tannins
  • 2.4.2 Quantitative determination
  • 2.4.2.1 Alkaloids determination
  • 2.4.2.2 Saponin determination
  • 2.4.2.3 Tannin determination
  • 2.4.2.4 Flavonoid determination
  • 2.4.2.5 Estimation of total Phenolic content
  • 2.4.2.6 Reducing Power
  • CHAPTER THREE
  • 3.0 Results
  • 3.1 Alkaloid determination
  • 3.2 Saponin determination
  • 3.3 Tannin determination

Chapter Four

  • 4.0 Discussion and Conclusion
  • 4.1 Discussion
  • 4.2 Conclusion
  • References

List Of Tables


  • Table 1 – Showing the qualitative and phytochemical analysis of Ocimum grattissimum
  • Table 2 – Showing the tannic acid absorbance
  • Table 3 – Showing the quercetin absorbance
  • Table 4 – Showing the absorbance of reducing power
  • Table 5 – Showing the determination of total Phenolic content
  • Table 6 – Showing the phytochemical analysis of hexanolic extract of ocimum gratissimum
  • Table 7 – Bioactivity of compounds detected through GC-MS screening of hexane extract of scent leaves

List Of Figures


  • Figure 1 – Graph showing the tannic acid standard curve
  • Figure 2 – Graph showing the quercetin standard curve
  • Figure 3 – Graph showing the determination of reducing power
  • Figure 4 – Graph showing the determination of total phenolic content

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