Isolation Of Bacteria From Orange Juice From Three (3) Commercial Company

Project and Seminar Material for Science Laboratory Technology SLT

Isolation Of Bacteria From Orange Juice From Three (3) Commercial Company


Abstract


Bacteriological examination of orange juice from three (3) commercial companies was carried out. The dilution method was employed using nutrient agar and macconkey agar for bacterial isolates five (5) samples of three different orange juice each were bought for the analysis isolated organism were Staphylococcus aureus, Escherichia coli and Streptococcus spp. The total bacterial mean count of frutta was 13 cfu, fumma was 12 cfu and chivita was 10 cfu. The highest bacterial isolate was Staphylococcus arueus with 20 cfu (53%) followed by Esherichia coli with 14 (37%) and the least wasStreptococcus spp with 4 (10%). Micro-organisms associated with orange juice spoilage are not too much to cause harm to human consumption to increase and improve the hygienic process used.


Chapter One


1.0 Introduction

Orange juice is a popular beverage made from the fresh orange fruit, by addition of water to the concentrate, desiccation and subsequent reconstitution of dried juice (Jawaad 2007). The term “orange juice” is also used both colloquially and commercially. Orange juice is one of the commodities traded on the New York Board of Trade. Hoard (1996) states that Brazil is the largest producer of orange juice in the world followed by the USA. Orange juice is made by sequencing the fruit on a special instrument called “Juicer” or a “squeezer”. The juice is collected in a small tray underneath. This is mainly done in the home and in industries. It is done on much larger scale.

Orange juice is gotten from the species of orange especially the sweet orange (Citrus sinensis). It is the most commonly from tree fruit in the world. Orange is a hybrid of ancient cultivated origin, possibly between pomelo (citrus maxima) and mandarin (Citrus reticulate). Wilson (2008) states that statistical data revealed that in Nigerian, between 1971-1976, diagnosed cases of acute food poisoning were estimated to be over 12000 persons. He continues to explain that it is important to note that food poisoning bacteria do not usually alter the appearance, taste or smell of the orange juice, therefore it is impossible by visual inspection to detect whether or not the orange juice is dangerously contaminated which makes this very hazardous to health when consumed, therefore bacteriological examination is expedient.

Some of the micro organisms that can contaminate orange juice and cause poisoning are Salmonella typhi, Staphilocccus auveus,Mycobacterium tuberculosis, Escherichia coli, Clostridium perfringerea, Vibrio cholerae, Bacillus cerus, Listeria, Monocytogenesand the host of others (Stanley 2007).


1.1 Statement Of Problem

Low standard of hygiene and improper processing methods that is inadequate GMP (Good manufacturing process) result to the presence of bacteria in ready to drink processed orange juice which could cause poisoning and sometimes death. Therefore it is important that the bacteria associated with orange juice contamination be examined, detected and the possible sources of contamination determined.


1.2 Aims Of The Study

  1. To isolate bacteria from orange juice from three commercial companies
  2. To identify the bacterial types
  3. To determine the bacterial load of each bacterial type.
  4. To determine the bacterial loads of the orange juice from three commercial companies sampled.

1.3 Limitation Of The Study

This work is limited to only orange juice from three commercial companies. Also the microbial examination is limited to microscopy, viable plate count, endospore staining and grain staining due to financial constraint in purchasing other selective media that would have enhanced the smooth running of this work. Another limiting factor is the non availability of some equipment like electron microscope required for practical work.


1.4 Significance Of The Study

The result of this project work would show if the bacterial loads of the three commercial companies producing orange juice were high or low. The results would equally show if the bacterial types were pathogenic. If the bacterial loads were high and pathogenic then hygienic practices were not observed and should be introduced. But if bacterial loads were low and bacterial types were not pathogenic, then hygienic practices were observed and should be encouraged.


Isolation Of Bacteria From Orange Juice From Three (3) Commercial Company


Project Material Download

3,000 Naira


The complete material will be sent to you in just 2 steps.

Quick & Simple…


Step One Purchase

Make payment of ₦3,000: through USSD Transfer, Bank Mobile App, ATM Transfer, or POS Transfer to:

Access Bank PlcAccount No.: 0811003731
Name: Samphina Academy
Account Type: Current

Or Click Here to pay with Debit Card

FOR CLIENTS OUTSIDE NIGERIA:
Click Here to pay with Debit Card ($15)
GHANA – Make Payment of 60 GHS to MTN MoMo, 0553978005, Douglas Osabutey 

  PAY WITH CRYPTOCURRENCY


Step Two Purchase

Send the following details through Text Message or WhatsApp Messenger | +234-8143831497

  • Payment Details 
  • Email Address 
  • Isolation Of Bacteria From Orange Juice From Three (3) Commercial Company

The complete material will be sent to your email address after receiving your payment information | T & C Apply


  Contact Our Help Desk


You may also like:

⚠️ Need a different topic? Perform a quick search



Get A Complete Business Plan For Any Business In Nigeria

Business Plan for Businesses in Nigeria

  Business Plans in Nigeria


Disclaimer


This research material “Isolation Of Bacteria From Orange Juice From Three (3) Commercial Company” is for research purposes and should be used as a guide in developing your research project / seminar work. For no reason should you copy word for word (verbatim) as samphina.com.ng will not be liable for any who copied the material.

The aim of providing this material is to reduce the stress of moving from one school library to another all in the name of searching for research materials. This service is legal because, all institutions permit their students to read previous projects, books, articles or papers while developing their own works. According to Austin Kleon “All creative work builds on what came before”.

samphina.com.ng is only providing this material “Isolation Of Bacteria From Orange Juice From Three (3) Commercial Company” as a reference for your research. The paper should be used as a guide or framework for your own paper. The contents of this paper should be able to help you in generating new ideas and thoughts for your own research. Use it as a guidance purpose only.

Samphina Academy

Samphina Academy is an Online Educational Resource Center that is aimed at providing students with quality information and materials to aid them in succeeding in their academic pursuit.