An Investigation Into The Spoilage Of Some Selected Fruits Of Eastern Nigerian (Paw Paw, Banana, Orange)

Project and Seminar Material for Science Laboratory Technology SLT

An Investigation Into The Spoilage Of Some Selected Fruits Of Eastern Nigerian (Paw Paw, Banana, Orange)


Abstract


Microorganisms associated with spoilage of fruits were studied using standards microbiological methods. The analysis was done on banana, pawpaw and orange using various media (Nutrient, Mac conkey and Sabroud agar). It showed that E. Coli (28.6%) were the most predominant bacterial isolates associated with the spoilage of selected fruits, in Easter part of Nigeria. This was followed by Enterobacter Spp (21.4%), Staphylococcus aureus (14.3%), Ermwinia spp (14.3%) and Pseudomonas Spp (14 -3%) while Samonella Spp (7.1%) was the least predominant.

The fungi isolate obtained were Aspergillus Niger (25.0%), Aspergillus Fumigatus (37.5%) Rhizopus Stolonifer (37 – 5%) and A. niger to be the least predominant. No protozoa or viruses were found to be associated with the spoilage of selected fruits. The frequence occurrence show that the bacterial isolates were the most predominant (63.6%) than fungi isolates. The method used for the analysis were streaking plate method. The presence of indicator and other organisms examined in this study is of special concern and perhaps the greatest danger associated with food for human consumption is contaminated by human excrement. Therefore the need for microbial assessment of fruits before consumption is highly need.


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