Extraction Of Neem Seed Oil

Project and Seminar Material for Science Laboratory Technology SLT

Extraction Of Neem Seed Oil


Extraction of neem seed oil was carried out using solvent extraction (n-hexane). 200g of neem seed yield 40ml of oil which is 80% yield. The neem oil is dark brown in colour, it has a strong odour that is said to combine the odours of palm kernel and garlic. The extraction of neem seed oil occurred in batches using solvent (n-hexane) also soxhlet extractor was used. The sample was ground and 200g was weighed out and soaked with 1000ml of n-hexane for 24 hours. With the help of butchner funnel and the vacuum pump, the solvent (n-hexane and neem seed oil) was filtered off from the sample leaving the residual in the buchner funnel.

As the solvent was being heated, it vapourized and passed through the condenser and then condensed and trickled down on the thimble containing the neem seed. The reaction occurred in the thimble. The n-hexane now extracts the essential oil in the sample and also other impurities like resins and waxes. When the liquid level filled the body of the extraction, it was then automatically siphoned back to the flask. This extract took about three hours for complete extraction. At the end, the set was dismantled and the sample changed. At the end of the extraction using 200g of neem seed and 100ml of n-hexant, 40m of neen seed oil was obtained.

Chapter One


Neem oil is a vegetable oil pressed from the fruits and seeds of the neem (Azadirachta indica) an evergreen tree which is endemic to the Indian subcontinent and has been introduced to many other areas in the tropics. It is the most important of the commercial available products of neem for organic farming and medicines.

Neem oil varies in colour; it can be golden yellow, yellowish brown, reddish brown, dark brown, greenish brown, or bright red. It has a rather strong odour that is said to combine the odours of peanut and garlic. It is composed mainly of triglycerides and contains many triterpenoid compounds, which are responsible for the bitter taste. It is hydrophobic in nature; in order to emulsify it in water for application purposes, it must be formulated with appropriate surfactants.

Neem oil is not used for cooking purposes. In India, it is used for preparing cosmetics (soap, hair products, body hygiene creams, hand creams) and in Ayurvedic, Unani and folklore traditional medicine, in the treatment of a wide range of afflictions. The most frequently reported indications in ancient Ayurvedic writings are skin diseases, inflammations and fevers, and more recently rheumatic disorders, insect repellent and insecticide effects.

Neem (Azadirachta indica A, Juss) is one of the very few trees known in the Indian subcontinent. This tree is belonged to Meliceae family, and grows rapidly in the tropic and semi-tropic climate. It is also observed that this tree could survive in very dry and arid conditions.

Neem tree is an evergreen tree, but it may become leafless for a short period in certain conditions. Neem tree also found in Indonesia in several areas such as Bali, Lombok, West Java, East Java (Situbondo, Ngawi), Central Java, and Nusa Tenggara Barat. All parts of Neem plant such as leaves, bark, Flower, fruit, seed and root have advantages in medical treatment and industrial products. Its leaves can be used as drug for diabetes, eczema and reduce fever. Barks of Neem can be used to make toothbrush. Neem roots has an ability to heal diseases and against insects (Khraisha, 2000) however, this tree is to very popular in Indonesia because it is only used as canopy tree. Neem seed is a part of “Neem tree which has high concentration of oil. Neem oil is widely used as insecticides, lubricant, drugs for variety of diseases such as diabetes and tuberculosis (Johnson, 1996). This oil could also prolong leather goods when it is applied on them.

There are several methods to obtain Neem oil from the seeds like mechanical pressing, supercritical fluid extraction, and solvent extraction. Mechanical extraction is the most widely used method to extract Neem oil from “Neem seed. However, the oil produced with this method usually has a low price, since it turbid and contains a significant amount of water and metals contents. Extraction using supercritical fluid, the oil produced has very high purity; however the operating and investment cost is high. Extraction using solvent has several advantages. It gives higher yield and less turbid oil than mechanical extraction, and relative low operating cost compared with supercritical fluid extraction.

1.1 Objective of the Study

This project work is aimed at extraction of oil from This project work is aimed at extraction of oil from neem seed.

1.2 Statement of Problems

In this our present day. The demand for self-reliance is high. Alternatives to exotic methods and equipments should be unprovidedly available.

In industry, commerce, and the society at large, essential oils are good sources of raw materials, revenue and flavouring respectively. Thousands of products are produced using oil and they include: cosmetics, drugs soaps and many others.

It has been found out that there is high demand of these oils in contrast to the poor availability of the products and so answers should be created and methods of extraction of these oils employed to obtain maximum yield and alleviate the problem.

Palm oil, groundnut oil and soya bean oil are the common oil used in Nigeria and all the edible oils and there is always a competition between food uses and industrial uses and this is a problem. An alternative non-edible oil will be of immense industrial significance and mean oil will meet this need when well exploited.

1.3 Hypothesis

Research hypothesis was carefully carried out to ensured adequate extraction of the neem seed oil from the neem seed

  1. HI: Solvent extraction is use for the extraction of the neem seed oil from the neem seed
    HO: Steam distillation is also use for the extraction of neem seed oil from the neem seed
  2. HI: Solvent extraction is better than steam distillation
    HO: Steam distillation is better than solvent extraction.

1.8 Limitations of Study

This project work is limited to the extraction of neem seed oil from neem seed. Finance is also a limiting factor during this project work.

Chapter Five

5.0 Conclusion and Recommendation

5.1 Conclusion

Agricultural commodities are shifting form exportation in their primary form to process form. This is aimed at increasing value adding to primary forms of commodities. Thus ensuring improved nutrition as well as creating job opportunities in rural areas.

In order to spread the take off of these cottage industries in most neem producing areas, small-scale farmers groups or co-operatives, comprising not less than 20 members can be assisted with credit facilities under the small scale enterprise scheme.

The study has show that the production of cashew from the oil extractor is a profitable venture. Cashew oil provides vitamin A. a necessary consistent on human diets. Its use will improve the nutritional health of the rural communities. In Brazil, the oil extracted is given some slight processing before being canned and sold. It is therefore, important to encourage extraction of the oil form cashew nut utilizing the locally fabricated equipment.

Support should be given to farmers and other local entrepreneurs under the small-scale enterprise scheme.

5.2 Recommendations

Oil should be extracted using soxhlet extractor and so efforts should be made by the management to obtain this equipment for use.

The steam distillation apparatus should be improved so effective extraction and quantification.

Oils are lital part of our society as applications could be used industrially for cosmetics, medications, repellants, flavouring and attractions etc.

The production of oil in large scale will also encourage self reliance. Oil should be used to avoid and also they should be converted to solid products to avoid the rapid of loss them.

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