Efficacy of Aqueous and Ethanol Extracts From Red Onions (Allium Cepa) as Alternatives to Haematoxylin and Eosin Stains

Medical And Health Science Project And Seminar Material

Efficacy of Aqueous and Ethanol Extracts From Red Onions (Allium Cepa) as Alternatives to Haematoxylin and Eosin Stains


Research Questions


  1. What are the staining efficacy of aqueous and ethanol extracts from red onions (Allium cepa) compared to traditional Haematoxylin and Eosin (H&E) stains?.

  2. What are the optimal extraction methods for obtaining red onion extracts with superior histological staining properties?.

  3. What are the reproducibility and consistency of staining outcomes using red onion extracts across different tissue types?.

  4. What are the potential antimicrobial and anti-inflammatory properties of red onion extracts and their impact on tissue preservation in histological specimens?.

Table of Content


  • Title Page
  • Certification
  • Dedication
  • Acknowledgement
  • Table of Content
  • Abstract

Chapter One

Introduction

  • 1.1 Background of the Study
  • 1.2 Statement of the Problem
  • 1.3 Objectives of the Study
  • 1.4 Research Questions
  • 1.5 Significance of the Study
  • 1.6 Scope of the Study
  • 1.7 Definition of Terms

Chapter Two

Literature Review

  • 2.1 Introduction
  • 2.2 Histological Staining Techniques
  • 2.2.1 Haematoxylin and Eosin Stains
  • 2.2.2 Alternative Staining Methods
  • 2.3 Allium Cepa (Red Onion) Extracts
  • 2.3.1 Chemical Composition
  • 2.3.2 Previous Applications in Medicine
  • 2.4 Previous Studies on Allium Cepa Extracts in Histology
  • 2.5 Summary

Chapter Three

Materials and Methods

  • 3.0 Introduction
  • 3.1 Research Design
  • 3.2 Sampling Technique
  • 3.3 Collection of Red Onion Samples
  • 3.4 Preparation of Aqueous and Ethanol Extracts
  • 3.5 Preparation of Histological Slides
  • 3.6 Staining Procedure
  • 3.7 Data Collection
  • 3.8 Data Analysis

Chapter Four

Results and Discussion

  • 4.1 Introduction
  • 4.1 Characteristics of Aqueous and Ethanol Extracts
  • 4.2 Comparison With Haematoxylin and Eosin Stains
  • 4.3 Histological Analysis
  • 4.4 Discussion of Findings
  • 4.5 Limitations of the Study

Chapter Five

Conclusion and Recommendations

  • 5.0 Introduction
  • 5.1 Summary of Findings
  • 5.2 Conclusion
  • 5.3 Recommendations for Further Research
  • References

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