The Effect Of Pterocarpus Mildbreadii Seed On Plasma HDL Cholesterol Of Albino Rat

Project and Seminar Material for Biochemistry

The Effect Of Pterocarpus Mildbreadii Seed On Plasma HDL Cholesterol Of Albino Rat


Abstract


This work was carried out to investigate the effects of pterocarpus mildbreadii seed on the HDL- level of albino rats.High density lipoprotein(HDL) is a fraction of cholesterol,which helps to transport cholesterol from peripheral of cell to the liver.High density lipoprotein is carried out to investigate cardio vascular diseases.Fresh oha seed (pterocarpus mildbreadii seed) was collected from Amokwe in udi local government Area Enugu state. They were dried at room temperature for about a month in an open laboratory space, groud into coarse form and weighed on an electrical weighing balance. 3% tween-80 was prepared by dissolving 3% tween -80 in 97ml of distilled water. The grounded pterocarpus mildbreadii was dissolve in the 3% tween-80.A total of sixteen wistar abino rats used for this analysis. The rats were divided into 4(four) groups. Group 1contain 4 rats and received 0.5 each of 3% tween-80. Group 2 contain 5 rats and were administered with 1400mg/kg b.w of pteridocarpus mildreadii solution Group 3 contain 3 rats and were administered with 2600mg/kg. b.w of pteridocarpus mildbreadii solution. Group 4 contain 4 rats and were administered with 500mg/kg. b.w. of Edmard MRT complex.


Table Of Content


  • Tittle page  i
  • Certification   ii
  • Dedication  iii
  • Acknowledgement iv
  • Abstract v
  • Table of content  vi
  • Table Of Content

Chapter One

  • 1.0 Introduction 1

Chapter Two

  • 2.0 Literature review  3
  • 2.1 lipids 4
  • 2.2 Classification of lipids  4
  • 2.3 Triglycerides 6
  • 2.4 Waxes 8
  • 2.5 Phospholipid 8
  • 2.6 Fatty acids 8
  • 2.7 Types of fatty acids 9
  • 2.8 Unsaturated fatty acids 10
  • 2.9 Essential fatty acids 11
  • 2.10 Steroids 11
  • 2.11 Cholesterol 12
  • 2.12 Dietary source & effect of Cholesterol  15
  • 2.13 Functions of Cholesterol in the body 17
  • 2.14 lipoprotein metabolism 19
  • 2.15 very low density lipoprotein (VLDL) 19
  • 2.16 low density lipoprotein 20
  • 2.17 Function of LDL 21
  • 2.18 High density lipoprotein (HDL) 21
  • 2.19 Clinical significance 22
  • 2.20 Fat soluble vitamin  23
  • 2.21 Vitamin A  24
  • 2.22 Vitamin D  25
  • 2.23 Vitamin E 26
  • 2.24 Vitamin K 27
  • 2 .25 lipid storage in plants 27
  • 2.26 lipid Body structure 27
  • 2.27 Unusual fatty acids in seed triacylglycerol 28
  • 2.28 Properties of lipids 29
  • 2.29 Physical properties 30
  • 2.30 Chemical properties 31
  • 2.31 Rancidity  32
  • 2.32 Roles of lipids  32
  • 2.32 Structure  33
  • 2.34 Insulation 33
  • 2.35 Nerve function 33
  • 2.36 Energy source  34
  • 2.37 Uses of oha seed (pterocarpus mildbreadii) 34

Chapter Three

  • 3.0 Material  35
  • 3.1 Plant seed  35
  • 3.2 Animals 35
  • 3.3 Equipments 36

Chapter Four

  • 4.1 Cholesterol activity 40

Chapter Five

  • 5.0 Discussion and conclusion 41
  • 5.1 Discussion 41
  • 5.2 Conclusion  41
  • Appendix 42
  • Reference 46

Chapter One


Introduction

Pterocarpus mildbreadii are trees with edible parts successfully budded, pterocarpus species (P.Soyauxii and P. mildbeadii are reported to have different patterns of leaf flush, one spontanous and the other intermitted making the first suitable for commercial production and the second the home production (Okafor 1978). Pterocarpus mildbreadii being the major case study both the vegetable and the seeds.

The vegetable is majorly used in our various home for cooking. Pterocarpus mildbreadii (Oha seed) are not consumed by the people from the eastern part of Nigeria. So it cannot be majorly stated the content of this oha seed.

The demand for vegetable oils as a result of diminishing source of oils and fats creates the needs for new sources as well as exploiting sources that are currently unexploited in order to supplement the existing ones (Minzangi et al. 2011).

As industrialists continues to rely mostly on the popular vegetable oil like coconut oil, soyabeans oil, ground nut oil, palm oil etc. for the preparation and production of their various products.


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