The Effect Of Ethanolic Extract Of Acanthus Montanus On Fasting Blood Sugar Levels Of Adult Wistar Rats

Project and Seminar Material for Biochemistry

The Effect Of Ethanolic Extract Of Acanthus Montanus On Fasting Blood Sugar Levels Of Adult Wistar Rats                                              


                                                     Abstract


This research work was carried out to determine the effect of Acanthus montanus on fasting blood sugar level of adult wistar rats. Twenty wistar rats (both male and female) weighing between 140g to 160g were used for this research project. They were acclimatized for 14 days after which the animals were grouped into four groups of five rats each. Each animal were administered based on their body weight. The dose given to animals in group A was 300mg/kg body weight of Acanthus montanus, Group B was given 500mg/kg body weight of Acanthus montanus, Group C was given 700mg/kg body weight of Acanthus montanus and group D (control group) was given distilled water. The administration lasted for thirteen days. A day before the animals were tested for fasting blood sugar level, food and water was withdrawn and they fasted for twelve hours. Blood samples were collected from the tail of the animals and the fasting blood sugar of each of the animals was tested.


Table Of Contents


  • Title page i
  • Certification page ii
  • Dedication iii
  • Acknowledgement iv
  • Abstract v
  • Table of content

Chapter One

  • 1.0 Introduction 1
  • 1.1 Plants
  • 1.2 Aim of Study

Chapter Two

  • 2.0 Literature Review
  • 2.1 The Uses of Medicinal Plants
  • 2.2 Acanthus Montanus
  • 2.3 Scientific Classification of Acanthus Montanus
  • 2.4 Fasting Blood Sugar

Chapter Three

  • 3.0 Materials and Methods
  • 3.1 Preparation of plants
  • 3.1.1 Chemicals, Reagents and Equipments
  • 3.2 Management of Animals
  • 3.3 Administration of Plant Extract
  • 3.3.1 Determination of Drug Dosage for Acanthus Monthanus
  • 3.3.2 Preparation of Stock Solution for Acanthus Montanus Ethanolic Extract
  • 3.3.3 Calculation of Administered Volume
  • 3.4 Sample Collection and Determination of Fasting Blood Sugar
  • 3.5 Statistical Analysis

Chapter Four

  • 4.0 Results
  • 4.1 Biochemical Parameters and Statistical Analysis

Chapter Five

  • 5.0 Discussion, Conclusion and Recommendation
  • 5.1 Discussion
  • 5.2 Conclusion
  • 5.3 Recommendation
  • References
  • Appendix

List Of Figures


  • Figure 1- Acanthus montanus
  • Figure 2- Graphical representation of Acanthus montanus on fasting blood sugar level

List Of Table


  • Table 1 – Grouping and administration of treatment
  • Table 2 – Showing the fasting blood sugar readings
  • Table 3 – Showing the effect of fasting blood sugar in the experiment and control group of adult wistar rats.

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