Determination Of Ascorbic Acid In Selected Fruits Mango, Orange And Pineapple Using Spectro-Photometric Method

Project and Seminar Material for Biochemistry

Determination Of Ascorbic Acid In Selected Fruits Mango, Orange And Pineapple Using Spectro-Photometric Method


Organic fruits are perceived to have a higher nutritional content than conventionally grown fruits, but currently, literature is rather ambiguous on the accuracy of this claim. Using vitamin C concentration as a nutritional marker, we found no clear or significant difference in the nutritional content between organically grown and conventionally grown fruit. The fruits analyzed were: oranges, mangoes and pineapple. Of the three types of fruits analyzed by cyclic voltammetry only, Orange demonstrated a significantly higher vitamin C concentration for organically grown versus conventionally grown fruit. Our results suggest that other factors are more influential on vitamin C levels than whether fruits are organically or conventionally grown. The detail about the comparative determination of ascorbic acid concentration in the three deferent kinds of the highest concentration of ascorbic acid among the fruits used than followed by pineapple and mango. The difference or variation of the result in all kinds of fruits used may also be due to the physical-chemical changes that eventually take place on the repining process of the fruits in case of any further research. It is recommended that people should be eating more fruits in order to meet up daily intake or vitamin C.

Chapter One

1.0 Introduction

A fruit is the dibble part of a plant that develops from flower. Many of the fruits grown in the fruits has grown in the wild for thousands of years. When people began to eat them as food they simply gathered the wild fruits and vegetables then about 11,000 years ago people began to plant fruit and vegetables to form the plants and to care for them. The formers experimented and grew new kinds of the wild fruits and vegetables (Huitray, 2000).

Fruits and vegetables found growing in one part of the world were taken by explorers and the fruits and vegetables were then planted and grown in many countries around the world. For example, it is believed that Christopher Columbus took pineapples to Spain from South America in the 1400s. In china apricots were grown 400s years ago and peaches over 2000 years ago. The Avacodois Naure to South America. It is not known for certain in where the lemon originated but India, Burma and Southern China are the most likely places. Pears grow wild in Britain is one country where olives first grow pineapples and nature to Brazil. Orange ones were grown in Holland and sold to other countries in the 1600s and were grown in Holland and sold to other countries in the 1600s and 1700s Ojokh-et al. (2005).

Fruits can be utilized in many ways and as such, have variedly of uses raging from food uses, medical and industrial uses. Fruits are generally known to belong to a class of foods that supply sugar mineral salts and vitamins (especially vitamin c and B complex vitamins (Aletor, 1993). Vitamins c is the simple sugar found in living tissues. It highest concentrations being in the adrenal cortex ad the eye. Stress and corticotrophin lead to a loss of vitamin c from the ordinal cortex. Fresh fruits and vegetables particularly black currents, citrus fruits barriers and been vegetable and the richest dietary sources or it may be synthetically prepared.

1.1 Statement of the Problem

These project is try to know the comparative determination of ascorbic acid (i.e. vitamin content in selected fruits juices. These intend get fruits i.e. Orange, Pineapple and mango. In Talata Mafara with these possible problems were therefore constrain in purchasing of these fruits especially mango.

1.2 Scope of the Study

This project work deals with the comparative and determination of vitamin C contents in fruits (Mango, Pineapple and Orange). This project will focus on how and what types of fruits have the highest vitamin c contents.

1.3 Aims and Objectives

These project or research is aimed at the following:

  1. To know the content of vitamin c in three selected fruits.
  2. To know the quantity of ascorbic acid to be absorb by human being.
  3. To also know the deficiencies accrue to excess or below intake of ascorbic acid more so to know the normal range required.
  4. To know the source require to get ascorbic acid.

1.4 Justification of the Study

In the modern society many people doesn’t know the important and content of ascorbic acid in fruits. This project will expatiate more on the important and the determination of ascorbic acid or required by the body.

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