A Comparative Study Of Antioxidant And Alpha Amylase Inhibitory Activities Of Methanol Extracts Of Calotropis Procera And Euphorbia Hirta Leaves

Project and Seminar Material for Biochemistry

A Comparative Study Of Antioxidant And Alpha Amylase Inhibitory Activities Of Methanol Extracts Of Calotropis Procera And Euphorbia Hirta Leaves


​Objectives of the Study


  1. To prepare methanol extracts of Calotropis procera and Euphorbia hirta leaves.

  2. To evaluate the antioxidant activity of the methanol extracts through assays such as DPPH radical scavenging activity, total phenolic content determination, and total flavonoid content determination.

  3. To assess the alpha amylase inhibitory activity of the methanol extracts using in vitro assays.

  4. To analyze and compare the phytochemical composition of the methanol extracts, identifying major bioactive compounds present in the extracts and relating them to the observed biological activities.

Table of Content


  • Title Page
  • Certification
  • Dedication
  • Acknowledgement
  • Table of Content
  • Abstract

Chapter One:

Introduction

  • 1.1 Background of the Study
  • 1.1.1 Importance of Antioxidants
  • 1.1.2 Role of Alpha Amylase Inhibitors
  • 1.1.3 Medicinal Properties of Calotropis procera
  • 1.2 Statement of the Problem
  • 1.2.1 Issues with Current Synthetic Treatments
  • 1.2.2 Need for Natural Alternatives
  • 1.3 Aim and Objectives of the Study
  • 1.3.1 Aim of the Study
  • 1.3.2 Objectives of the Study
  • 1.4 Justification
  • 1.4.1 Potential Health Benefits
  • 1.4.2 Contribution to Scientific Knowledge
  • 1.4.3 Basis for Future Research and Applications

Chapter Two:

Literature Review

  • 2.0 Introduction
  • 2.1 Overview of Antioxidants
  • 2.2 Mechanisms of Alpha Amylase Inhibition
  • 2.3 Pharmacological Properties of Calotropis procera
  • 2.4 Pharmacological Properties of Euphorbia hirta
  • 2.5 Comparative Studies on Plant Extracts
  • 2.6 Summary of Key Findings from Previous Research

Chapter Three:

Materials and Methods

  • 3.1 Plant Material Collection and Identification
  • 3.2 Preparation of Methanol Extracts
  • 3.3 Antioxidant Activity Assays
  • 3.3.1 DPPH Radical Scavenging Activity
  • 3.3.2 Total Phenolic Content
  • 3.3.3 Total Flavonoid Content
  • 3.4 Alpha Amylase Inhibition Assays
  • 3.4.1 In Vitro Assays
  • 3.4.2 Data Analysis
  • 3.5 Phytochemical Analysis
  • 3.5.1 Identification of Bioactive Compounds
  • 3.5.2 Relation to Biological Activities

Chapter Four:

Results and Discussion

  • 4.1 Antioxidant Activity of Methanol Extracts
  • 4.1.1 DPPH Radical Scavenging Results
  • 4.1.2 Total Phenolic and Flavonoid Contents
  • 4.2 Alpha Amylase Inhibitory Activity of Methanol Extracts
  • 4.2.1 Inhibitory Activity Results
  • 4.2.2 Comparative Analysis
  • 4.3 Phytochemical Composition
  • 4.3.1 Identified Compounds
  • 4.3.2 Correlation with Antioxidant and Inhibitory Activities
  • 4.4 Discussion of Findings
  • 4.4.1 Comparison with Previous Studies
  • 4.4.2 Implications for Therapeutic Use
  • 4.4.3 Limitations of the Study

Chapter Five:

Conclusion and Recommendations

  • 5.0 Introduction
  • 5.1 Summary of Key Findings
  • 5.2 Conclusion
  • 5.3 Recommendations for Future Research
  • 5.4 Practical Applications of Findings
  • References

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