Comparative Determination Of Protein Contents Of Breadfruit, Brown Beans And Soybeans

Project and Seminar Material for Science Laboratory Technology SLT

Comparative Determination Of Protein Contents Of Breadfruit, Brown Beans And Soybeans

Chapter One

1.0 Introduction

1.1 Background of the Study

Proteins are essential nutrients for the human body (Hermann, 2002). They are one of the building block of the body tissue, and also serve as a fuel source. As a fuel, protein contain 4kcal 7kj) per gram, just like carbohydrates and unlike lipids, which contain 9kcal (37kj) per gram. The most important aspect and defining characteristics of protein from a nutritional stand point is its amino acid composition (Laurence, 2000).

Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chain via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body (Genton, 2010). There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, lysine, leucine, threonine, tryptophan, methionine, isoleucine and histidine (Laurence, 2000). There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, sernine, asparagines and glutamic acid. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress (Laurence, 2000). These six are argnine, cysteine, glycine, glutamine, proline and tryrosine (Laurence, 2000). Sources of protein include grains, legumes and nuts, as well as animal sources such as meats, dairy products, fish and eggs (Young, 1994).

African breadfruit (Treculia Africana Decne) belongs to the mulberry family. Moracceae, which is of African origin but now grown in the most tropical and sub-tropical countries (Agu and Nwabueze, 2007). African breadfruit or wild jack fruit in some areas, is a neglected and under exploited tropical tree (Osuji and Owei, 2010).

According to Okonkwo and Ubani (2012), it is a common forest tree called various names among different tribes in Nigeria, such as “Ukwa” (Igbo), “afon” (Yoruba), “eyo” (Igala), “barafuta” (Hausa), “Ize” (Benin) and “edikang” (Efik). The tree crop is widely grown in the southern state of Nigeria where it serves as low cost meat substituent for poor families in some communities (Badifu and Akuba, 2001; Ugwu, et al, 2001). the plant produced large, usually round, compound fruit covered with pointed outgrowths and the seeds are buried in the spongy pulp of the fruits (Nwokolo, 1996). the seeds are seldom eaten raw but can be baked, roasted or fried before consumption, or they can be ground into flour in bakery products (Agu et al, 2007; Ijeh et al, 2010). African breadfruit seeds are highly nutritious and constitute a cheap source of vitamins, minerals, proteins, carbohydrates and fats.

Brown beans (Phaseolus Vulgaris) is a herbaceous annual plant grown worldwide for its edible dry seeds (Known as just ‘Beans”) or unripe fruit (Green beans). It’s leaf is also occasionally used as a vegetable and the straw as fodder. It’s botanical classification, along with other phaseolus species, is as a member of the legume family fabaceae, most of whose members acquire the nitrogen they require through association with rhizoidal, a species of nitrogen-fixing bacteria (Edet, 1982). Beans are grown in every continent except Antarctica. Brazil and India are the largest producers of dry beans, while china produces by far, the largest quantity of brown beans. Worldwide, 23 million tones of dry common beans and 17.1 billion tones of green were grown in 2010 (Philips, 2010). Similar to other beans, the brown beans is high in starch, protein and dietary fiber, and is an excellent source of iron, selenium, potassium, molybdenum, thiamine, vitamin B6 and folate (Paul, 1998) .

The soybean (Glycine max (L.) Merrill family Leguminosae, subfamily Papilionoidae) originated in Eastern Asia, probably in north and central china. It is believed that cultivated varieties were introduced into Korea and later Japan some 2000 years ago. Soybeans have been grown as food crop for thousands of years in China and other countries of East and South East Asia and constitute to this day, an important component of the traditional popular diet in these regions (William, 2003). Although the U.S.A and Brazil account today for the most of the soybean production of the world, the introduction of this crop to Western agriculture is quite recent. Soybeans are primarily, an industrial crop, cultivated for oil protein. Despite the relatively low oil content of the seed (about 20% on moisture-free basis), Soybeans are the largest single source of edible oil and account for roughly 50% of total oil seed production of the world (Singh, Nelson and Chung, 2008).

With each ton of crude soybean oil, approximately 4.5 tons of soybean oil meal with a protein content of about 44% are produced. For each ton of soybeans processed, the commercial value of the meal obtained usually exceeds that of the oil. Thus, soybean oil meal cannot be considered by-product of the oil manufacture. The soybean is, in this respect, an exception among oil seed (Shurtleff; Steenhuis and Spiers, 2013). It can be calculated that the quality of protein in the yearly world production of soybeans, if it could be totally and directly utilized for human consumption would be sufficient for providing roughly one third of the global need for protein (William, 2003). This makes the soybeans one of the largest potential source of dietary protein. However, the bulk of soybean oil meal is used in animal feed for the production of meat and eggs. Despite considerable public and commercial interest in soybean products as food, the proportion of soybean protein consumed directly in human nutrition is still relatively small (Smith, 1972).

1.2 Statement of Problem

It has been scientifically proven that every variety of beans is rich in protein and contains such amount of carbohydrates that is good for diabetic patients. This not the case with African breadfruit, while some say that it is highly protein ones other say it contains mostly carbohydrates. This controversy has created confusion to many especially diabetic patients who are cross road whether to keep eating it or not. This research is therefore aimed at setting the controversy.

1.3 Objective of the Study

The general objective of this present work is to determine and compare the protein content of breadfruit, brown beans and soybean.

The specific objectives are as follows;

  1. To determine the protein content of breadfruit.
  2. To determine the protein content of brown beans.
  3. To determine the protein content of soybeans.
  4. Comparison of protein content of breadfruit, brown beans and soybeans.

1.4 Significance of the Study

The research will be beneficial to the following;

  1. Diabetic patients
  2. Dieticians
  3. People in the health sector
  4. Vegetarians and
  5. The general public

1.5 Scope of the Study

This research work is limited to the comparative determination of protein content of breadfruit, brown beans and soybeans.

Chapter Five


The proximate or Weende analysis of feed (Breadfruit, Brown and Soybeans) is a quantitative method to determine different macronutrients in feed. Basically it is the partition of feed compounds into six categories by means of common chemical properties. The categories are moisture (crude water), crude ash (CA), crude protein (CP), ether extracts (fats or lipids; EE), crude fiber (CF) and nitrogen free extractives (NFE).

Possible error during moisture process which is loss of volatile fatty acid, and lactic acid in silage or any other fermented product. Most likely underestimate dry matter. This error is relatively small. Maillard product formation (protein: CHO complex.

The ash fraction contains all the mineral elements jumbled together. It would be more useful to know the amounts of different individual elements. This method consists of oxidizing all organic matter in a weighed sample of the material by incineration and determining the weight of the ash remaining. It is applicable to fish, fish products, and other materials with a low carbohydrate content. Note that the high temperature may cause the volatilization of certain elements (particularly K, Na, Cl, and P) and may also cause the mineral matter to melt and fuse.).

Possible errors and disadvantages in determination of protein experiment, this procedure assumes all nitrogen present in the sample are in protein form. This assumption is not necessarily true. Nitrogen could be in nucleic acid (RNA, DNA), urea. Different proteins need different correction factors because they have different amino acid sequences. The use of concentrated sulfuric acid at high temperatures poses a considerable hazard, as does the use of some of the possible catalysts. The technique is time consuming to carry-out.

Safety measure during determined of lipid content experiment. Ether has an extremely low flash point, must not have open flames nearby. Avoid inhaling ether vapors. Store ether in metal containers. Handle open containers (reagent cans and fat beakers) in a hood. Conduct the extractions in a well ventilated area. Make sure all ether is evaporated from the beakers before placing them in the oven to avoid a fire or explosion.

Possible errors during determination of lipid content experiment. This is the most unsatisfactory principle of the Proximate Analysis. Major problem is acid and base solubilize some of the true fiber (particularly hemicellulose, pectin and lignin). Cellulose too is partially lost. Hence, crude fiber underestimates true fiber.

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