Comparative Analysis Of Microbial Load Of The Main Water Production And Water Available To IMT Campus II Hostel

Project and Seminar Material for Environmental Science

Comparative Analysis Of Microbial Load Of The Main Water Production And Water Available To IMT Campus II Hostel


Samples of water from the different hostels in campus II and other source of water production namely Ajali-owa water, from bore hole 9th mile and spring water from GRA water board were water collected and analyzed using total plate count and multiple tube fermentation techniques coliform. The results shows no coliforms in production water from Ajali-owa and in GRA spring water reservaiour and no coliform in 9th mile bore hole water, 14 organism and 149 cfu/ml plate counts were obtained from tap water in IMT campus II hostel 7 coliform and Tocful / ml plate count from well water and 28 coliform and 280 cfu/ml plate count from spillage waste water. These results obtained show that tap water consumed in IMT campus II did not meet with NAFDAC and WHO standards. Preventive measures such as proper treatment of these water should be encourage and all pipes in campus II should be checked for leakages.

Table of Content

  • Title page
  • Certification
  • Dedication
  • Acknowledgement
  • Abstract
  • Table of content

Chapter One

1.0 Introduction

  • 1.1 Aims / objectives of the study
  • 1.2 Statement of problems
  • 1.3 Hypothesis
  • 1.4 Scope of limitation

Chapter Two

2.0 Literature Review

  • 2.1 The major group of biological Pollutions of water
  • 2.2 Source of water supply
  • 2.3 Source of water pollution
  • 2.4 Treatment of water sample
  • 2.5 International standard for drinking water
  • 2.6 Diseases from water

Chapter Three

3.0 Materials & Methods

  • 3.0 Materials
  • 3.1 Media preparation
  • 3.2 Collection of samples
  • 3.3 Collection of samples
  • 3.4 Methods of water analysis
  • 3.5 Determination of PH
  • 3.5.1 Determination of bacteria of the coliform
  • 3.5.2 Culture techniques
  • 3.5.3 Plate count technique
  • 3.5.4 Presumption coliform (multiple tube fermentation)
  • 3.5.4 Confirmatory test
  • 3.5.5 Completed test
  • 3.5.6 Test for indole
  • 3.5.7 Methyl red test
  • 3.5.8 Sugar test
  • 3.5.9 Oxidase test
  • 3.5.10 Catakase test
  • 3.5.11 The slide cougulase test
  • 3.5.12 Gram stain techniques
  • 3.5.13 Detection of faecal stredtococci
  • 3.5.14 Detection of escherichi coli test
  • 3.5.15 Organism suspected after gram staining

Chapter Four


Chapter Five


Chapter Six

6.0 Conclusion and Recommendation

  • 6.1 Conclusion
  • 6.2 Recommendation
  • References
  • Appendix

Chapter One


Water is a liquid mineral. It consist of two atoms of hydrogen combined with one atom of oxygen. Water is a tasteless, odourless and colourless mobile liquid except in large volume where it appears blue. It has a melting point of OoC (320f) and a boiling point of 1000C (2120f). water is a basic necessity of life, in the absence higher animals survive only but a few hours or days. It has several uses such as washing cooking, food processing ,swimming, and among others. Out of these uses, drinking of water seems to be the most sensitive as it could have a direct deleterious impact on health of human beings.

Therefore, drinking water should be potable free diseases, or toxic substance (Beren 1991) .

Water is portable when it is colourless. Odourless, or tasteless and also free from poisonous, corroding, staining substances as well as disease casuiong oirganisms (Cruich 1972). The problem of providing safe and adequate water is as old as life on earth. Water can be made unifit or unsafe for drinking it contamination occurs either by nature or activities of human beings.

Perhaps the greatest danger associated with drinking water is contamination by human excrement (Forest 1979). In most cases, the pollution/contamination is hardly severe and is not particularly detrimental to health.
Thus a few substances and microbes that are health hazard do occur in water, and can cause illness or even death. From microbiological point of view, the pathogens most frequently transmitted through water supply ranges from ultrasmall virus to the microspic bacteria and relatively large cysts entemoeba instolytica. These organism cense infectrons of intestinal trach, when consumed censing amebic dysentery, and Gardia lamblia is a protozoa responsible for Giadiasis. Defective plumbing for example was the cause of out break during the world fair in Chicago 1933 (Garelick 1987) water borne disease cause acute diarrhoe, often last 2-3 months. The most common water borne disease can be by bacteria includes typhoid fever, paratyphoid. Asiatic cholera and bacteria dysentery.
Other disease that may be transmitted by water include brucellosis, shige /losis, these disease are consuming.

Untreated water (Hetolett 1921) various countries of the world and the world Health organisation (WHO) has set up standards for water quality against which the level of pollution can be assessed. The world Health organization (WHO) National Agency for food and Drug Administration control (NAFDAC), standard for potable water include the following, that in 100ml of water coliform bacteria should be absent, total plate count of bacteria growth should be 100 CFU/ML of water. Hence the sanitary Quality of water should not be over looked since contaminated water can cause health hazards when consumed.

According to guideline for drinking water quality of WHO (1998)


World Health Organisation Standard for Drinking Water
PH5.0 – 9.0
COLOUR15 Hazen unit
Dissolved solids500 mg/l
Total Hardness500 mg/l
Calcium75 mg/l
Iron0.3 mg/l
Chloride200 mg/l
Nitrate45 mg/l
Coliform10 colonies

1.1 Aims / Objectives of the Study

The objective of this projects is to make comparative analysis of Enugu state water cooperation provide against supplies.

To identify contamination microbial advice on measures to control pollution of water bodies etc.

1.2 Statement of Problems

The treated water produced by Enugu state water co-operation could be contaminated by defective plumbing which cause the rush of water out of the pipe forming pull mixed with sewage matters. The pull eventually suck back through the burst pipe into the pipe line being distributed to various location and collected as drinking water.

1.3 Hypothesis

H.O: there is difference in the microbial load of water from Enugu state water operation at point of production against the load as the point of supply and consumption.

HI: There is statistical differences between the microbial load of water from the Enugu state water co-operation as the production point against the load as supply and consumption point.

1.4 Scope of Limitation

The project is set to identify only microbial pollutants the method of microbial analysis is limited to the techniques and materials available in our laboratory as against high tech equipment which may be found else where.

Chapter Six

Conclusion and Recommendation

6.1 Conclusion

Since water is vital in our everyday activities both industrially and domestically, the institution should do something in order to provide good portable water to the students and by so doing the spread of water borne disease ad other deadly diseases which could be contacted by the consumption of polluted/contaminated water will be reduced, personal cleanliness should also be maintained so that the students will be healthy. Provision of water should be of course a package deal involving the use of lareful plan for the disposal system of waste water and effluent any how along the surrounding in the hostels.

6.2 Recommendation

The plausible source of the contamination in the water bodies surveyed must be contamination from burst pipes especially if the bursts are in a pool of sewage / used water. This is so because production at site are free from coliform and other microbes. It is therefore recommended that, the water is not portable. The water should be treated before use as table water or drinking water.

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