Antibacterial Activity Of Honey On Staphylococcus Aureus Escherichia Coli And Streptococcus

Project and Seminar Material for Microbiology

Project and Seminar Material for Microbiology


Abstract


Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms.
The result revealed that the two honey samples have heavy antibacterial activities against the test organisms and zones of inhibition were obtained showing high antibacterial activity. The antibacterial activity increased with increase in the concentrations and honey from Nsukka produced a high antibacterial activity (clearer zone) on staphylococcus aureus and Escherichia coli at all concentration and moderately for streptococcus pyogens. The use of honey as a therapeutic substance has been rediscovered by the medical profession on more recent times, and it is gaining acceptance as an antibacterial agent for the treatment of ulcers and bed sores, and other infections resulting from burns and wounds


Chapter One


1.0 Introduction

Infections and other health related problems have been of great concern to human beings and chemotherapy is the main approach in the treatment of such conditions. Investigation into the microbial flora of wound began in the late 19th century and since then; improvements in techniques have facilitated the recovery, identification and enumeration of a wide variety of microbial species. Most wounds support relatively stable polymicrobial communities (Bowkler, et.al; 2001) often without signs of clinical infection (Hansson,et al; 1993).

However, potential pathogens may be present and the delicate balance between colonized wound and an infected wound depends on the interplay of complex host


Antibacterial Activity Of Honey On Staphylococcus Aureus Escherichia Coli And Streptococcus


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