Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel

Project and Seminar Material for Biochemistry

Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel


Abstract


The study was carried out to investigate the activities respiratory complex IV in the liver of normal and protein undernourished wistar rats fed with cassava peel. Twenty weaning male rats were grouped into four groups, five rats for each. Group A were fed with normal diets, Group B were fed with low protein diets containing 5% casein, Group C were fed with normal diets and coconut oil and Group D were fed with low protein diet and coconut oil. After feeding for a period of six weeks, the rats were sacrificed using cervical dislocation. The liver was harvested and homogenized in iced cold buffer and centrifuged at 4000rpm for 15 minutes. The pellets were discarded and the supernatant was stored.

It was observed from this study that consumption of low protein diets significantly reduced the activities of cytochrome oxidase in the rat liver when compared to normal diet. However, the activities of cytochrome oxidase were significantly reduced in the rat liver when the rats were fed with normal and cassava peel diets compared to normal diet only. Also, low protein and cassava peel diets significantly reduced the activities of cytochrome oxidase in the rat liver when compared to normal diet.

It was observed from this study that in the rat liver, the activities of cytochrome oxidase was significantly increased when the rats were fed with normal and cassava peel diets compared to low protein diet only. However, when the rats were fed with low protein diet and cassava peel, the diets significantly increased the activities of cytochrome oxidase compared to both normal and cassava peel and low protein diet only. This study shows that consumption of cassava peel significantly reduced the level of normal diets while it significantly increased the level of low protein diets.


Table Of Contents


  • Title page i
  • Certification page ii
  • Dedication iii
  • Acknowledgement iv
  • Abstract v
  • Table of content

Chapter One

  • 1.0 Introduction
  • 1.1 Aim of Study

Chapter Two

  • 2.0 Literature review
  • 2.1 Cassava peel
  • 2.2 Cyanide
  • 2.3 Respiratory Electron Transport Chain
  • 2.3.1 The sequence of electron transport chain
  • 2.3.2 Mitochondria
  • 2.4 Cytochrome oxidase
  • 2.5 Protein undernutrition
  • 2.6 Liver
  • 2.6.1 Synthesis
  • 2.6.2 Liver disease and blood supply

Chapter Three

  • 3.0 Materials and Methods
  • 3.1 Materials
  • 3.1.1 Chemicals and reagents
  • 3.2 Methods
  • 3.2.1 Animal managements
  • 3.2.2 Feed composition
  • 3.2.3 Body weight determination
  • 3.2.4 Animal sacrifice
  • 3.2.5 Isolation of mitochondria
  • 3.3 Determination of complex IV (Cytochrome oxidase activity
  • 3.4 Protein determination

Chapter Four

  • 4.0 Results

Chapter Five

  • 5.0 Discussion and Conclusion
  • 5.1 Discussion
  • 5.2 Conclusion
  • References
  • Appendix.

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